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biotinylated protein  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated protein
    Genetic knockout (KO) of inducible nitric oxide synthase (iNOS) results in a substantial reduction in the S-nitrosylation (SNO) of mitochondrial proteins . A-B. The images and the quantitated values of Western blots showing the total abundance of the representative mitochondrial proteins of each electron transport chain complex, in the heart tissues of wild-type (WT), VCP transgenic (TG), and VCP TG/iNOS KO mice. GAPDH used as a loading control in total protein extracts. C-D . The representative images and quantitated values of Western blots showing the SNO level of these proteins by testing the purified <t>biotinylated</t> proteins from the same heart tissues. *,p < 0.05, **, p < 0.01 vs. WT, ##: p < 0.01 vs. VCP TG mice.
    Biotinylated Protein, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-2001-1/Biotinylated+Protein+A%2C+S%2E+aureus/pmc11061752-96-1-10
    Average 93 stars, based on 134 article reviews
    biotinylated protein - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Valosin-containing protein acts as a target and mediator of S-nitrosylation in the heart through distinct mechanisms"

    Article Title: Valosin-containing protein acts as a target and mediator of S-nitrosylation in the heart through distinct mechanisms

    Journal: Redox Biology

    doi: 10.1016/j.redox.2024.103166

    Genetic knockout (KO) of inducible nitric oxide synthase (iNOS) results in a substantial reduction in the S-nitrosylation (SNO) of mitochondrial proteins . A-B. The images and the quantitated values of Western blots showing the total abundance of the representative mitochondrial proteins of each electron transport chain complex, in the heart tissues of wild-type (WT), VCP transgenic (TG), and VCP TG/iNOS KO mice. GAPDH used as a loading control in total protein extracts. C-D . The representative images and quantitated values of Western blots showing the SNO level of these proteins by testing the purified biotinylated proteins from the same heart tissues. *,p < 0.05, **, p < 0.01 vs. WT, ##: p < 0.01 vs. VCP TG mice.
    Figure Legend Snippet: Genetic knockout (KO) of inducible nitric oxide synthase (iNOS) results in a substantial reduction in the S-nitrosylation (SNO) of mitochondrial proteins . A-B. The images and the quantitated values of Western blots showing the total abundance of the representative mitochondrial proteins of each electron transport chain complex, in the heart tissues of wild-type (WT), VCP transgenic (TG), and VCP TG/iNOS KO mice. GAPDH used as a loading control in total protein extracts. C-D . The representative images and quantitated values of Western blots showing the SNO level of these proteins by testing the purified biotinylated proteins from the same heart tissues. *,p < 0.05, **, p < 0.01 vs. WT, ##: p < 0.01 vs. VCP TG mice.

    Techniques Used: Knock-Out, Western Blot, Transgenic Assay, Control, Purification

    Related Articles

    Saline:

    Article Title: NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection.
    Article Snippet: .. Granville; Shen et al.44 N/A Dispase II Roche Cat# 4942078001 Collagenase D Roche Cat# 11088866001 Dnase I Roche Cat# 10104159001 fetal bovine serum Corning Cat# 35-010-CV DMEM, high glucose Gibco Cat# 11965-092 RPMI 1640 Gibco Cat# 11875-093 Dulbecco0s Phosphate Buffered Saline (DPBS) Sigma-Aldrich Cat# D8537-500ML Phosphate Buffered Saline Powder Sigma-Aldrich Cat# P3813 Penicillin-Streptomycin Gibco Cat# 15140-122 bovine serum albumin Sigma-Aldrich Cat# A9418-10G normal goat serum Jackson Immunoresearch Cat# 005-000-121 RRID: AB_2336990 Normal donkey serum Jackson Immunoresearch Cat# 017-000-121 RRID: AB_2337258 Critical commercial assays Mouse Granzyme B DuoSet ELISA kit R&D Cat# DY1865-05 Mouse IFN-g DuoSet ELISA kit R&D Cat# DY485-05 Mouse IL-18 ELISA kit MBL Cat# 7625 Avidin/ Biotin Blocking Kit Vector Laboratories Cat# SP-2001 RRID:AB_2336231 Human Granzyme B DuoSet ELISA kit R&D Cat# DY2906-05 RRID:AB_2893368 Human Total IL-18 DuoSet ELISA kit R&D Cat# DY318-05 Experimental models: Cell lines Vero Cells (CCL81) ATCC CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Mouse: B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson Laboratory Cat# 022071; RRID:IMSR_JAX:022071 Mouse: Ncr1-Cre Provided by W. Yokoyama; Narni-Mancinelli et al.91 N/A Mouse: Il-18r1flox/flox Provided by R. A. Flavell; Nowarski et al.92 N/A Mouse: B6.129S2-Gzmbtm1Ley/J Provided by T. J. Ley; Pham et al.55 RRID:IMSR_JAX:002248 Mouse: C57BL/6-Prf1tm1Sdz/J Jackson Laboratory Cat# 002407; RRID:IMSR_JAX:002407 Software and algorithms Image J Fiji NIH https://imagej.net/software/fiji/ NDP 2.0 Hamamatsu https://www.hamamatsu.com/jp/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html GraphPad Prism GraphPad Software http://www.graphpad.com/ scientific-software/prism/ FlowJo BD https://www.flowjo.com/solutions/flowjo Cell Reports 42, 112410, April 25, 2023 15 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection.
    Article Snippet: .. Granville; Shen et al.44 N/A Dispase II Roche Cat# 4942078001 Collagenase D Roche Cat# 11088866001 Dnase I Roche Cat# 10104159001 fetal bovine serum Corning Cat# 35-010-CV DMEM, high glucose Gibco Cat# 11965-092 RPMI 1640 Gibco Cat# 11875-093 Dulbecco0s Phosphate Buffered Saline (DPBS) Sigma-Aldrich Cat# D8537-500ML Phosphate Buffered Saline Powder Sigma-Aldrich Cat# P3813 Penicillin-Streptomycin Gibco Cat# 15140-122 bovine serum albumin Sigma-Aldrich Cat# A9418-10G normal goat serum Jackson Immunoresearch Cat# 005-000-121 RRID: AB_2336990 Normal donkey serum Jackson Immunoresearch Cat# 017-000-121 RRID: AB_2337258 Critical commercial assays Mouse Granzyme B DuoSet ELISA kit R&D Cat# DY1865-05 Mouse IFN-g DuoSet ELISA kit R&D Cat# DY485-05 Mouse IL-18 ELISA kit MBL Cat# 7625 Avidin/ Biotin Blocking Kit Vector Laboratories Cat# SP-2001 RRID:AB_2336231 Human Granzyme B DuoSet ELISA kit R&D Cat# DY2906-05 RRID:AB_2893368 Human Total IL-18 DuoSet ELISA kit R&D Cat# DY318-05 Experimental models: Cell lines Vero Cells (CCL81) ATCC CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Mouse: B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson Laboratory Cat# 022071; RRID:IMSR_JAX:022071 Mouse: Ncr1-Cre Provided by W. Yokoyama; Narni-Mancinelli et al.91 N/A Mouse: Il-18r1flox/flox Provided by R. A. Flavell; Nowarski et al.92 N/A Mouse: B6.129S2-Gzmbtm1Ley/J Provided by T. J. Ley; Pham et al.55 RRID:IMSR_JAX:002248 Mouse: C57BL/6-Prf1tm1Sdz/J Jackson Laboratory Cat# 002407; RRID:IMSR_JAX:002407 Software and algorithms Image J Fiji NIH https://imagej.net/software/fiji/ NDP 2.0 Hamamatsu https://www.hamamatsu.com/jp/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html GraphPad Prism GraphPad Software http://www.graphpad.com/ scientific-software/prism/ FlowJo BD https://www.flowjo.com/solutions/flowjo Cell Reports 42, 112410, April 25, 2023 15 ..

    Avidin-Biotin Assay:

    Article Title: NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection.
    Article Snippet: .. Granville; Shen et al.44 N/A Dispase II Roche Cat# 4942078001 Collagenase D Roche Cat# 11088866001 Dnase I Roche Cat# 10104159001 fetal bovine serum Corning Cat# 35-010-CV DMEM, high glucose Gibco Cat# 11965-092 RPMI 1640 Gibco Cat# 11875-093 Dulbecco0s Phosphate Buffered Saline (DPBS) Sigma-Aldrich Cat# D8537-500ML Phosphate Buffered Saline Powder Sigma-Aldrich Cat# P3813 Penicillin-Streptomycin Gibco Cat# 15140-122 bovine serum albumin Sigma-Aldrich Cat# A9418-10G normal goat serum Jackson Immunoresearch Cat# 005-000-121 RRID: AB_2336990 Normal donkey serum Jackson Immunoresearch Cat# 017-000-121 RRID: AB_2337258 Critical commercial assays Mouse Granzyme B DuoSet ELISA kit R&D Cat# DY1865-05 Mouse IFN-g DuoSet ELISA kit R&D Cat# DY485-05 Mouse IL-18 ELISA kit MBL Cat# 7625 Avidin/ Biotin Blocking Kit Vector Laboratories Cat# SP-2001 RRID:AB_2336231 Human Granzyme B DuoSet ELISA kit R&D Cat# DY2906-05 RRID:AB_2893368 Human Total IL-18 DuoSet ELISA kit R&D Cat# DY318-05 Experimental models: Cell lines Vero Cells (CCL81) ATCC CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Mouse: B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson Laboratory Cat# 022071; RRID:IMSR_JAX:022071 Mouse: Ncr1-Cre Provided by W. Yokoyama; Narni-Mancinelli et al.91 N/A Mouse: Il-18r1flox/flox Provided by R. A. Flavell; Nowarski et al.92 N/A Mouse: B6.129S2-Gzmbtm1Ley/J Provided by T. J. Ley; Pham et al.55 RRID:IMSR_JAX:002248 Mouse: C57BL/6-Prf1tm1Sdz/J Jackson Laboratory Cat# 002407; RRID:IMSR_JAX:002407 Software and algorithms Image J Fiji NIH https://imagej.net/software/fiji/ NDP 2.0 Hamamatsu https://www.hamamatsu.com/jp/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html GraphPad Prism GraphPad Software http://www.graphpad.com/ scientific-software/prism/ FlowJo BD https://www.flowjo.com/solutions/flowjo Cell Reports 42, 112410, April 25, 2023 15 ..

    Blocking Assay:

    Article Title: NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection.
    Article Snippet: .. Granville; Shen et al.44 N/A Dispase II Roche Cat# 4942078001 Collagenase D Roche Cat# 11088866001 Dnase I Roche Cat# 10104159001 fetal bovine serum Corning Cat# 35-010-CV DMEM, high glucose Gibco Cat# 11965-092 RPMI 1640 Gibco Cat# 11875-093 Dulbecco0s Phosphate Buffered Saline (DPBS) Sigma-Aldrich Cat# D8537-500ML Phosphate Buffered Saline Powder Sigma-Aldrich Cat# P3813 Penicillin-Streptomycin Gibco Cat# 15140-122 bovine serum albumin Sigma-Aldrich Cat# A9418-10G normal goat serum Jackson Immunoresearch Cat# 005-000-121 RRID: AB_2336990 Normal donkey serum Jackson Immunoresearch Cat# 017-000-121 RRID: AB_2337258 Critical commercial assays Mouse Granzyme B DuoSet ELISA kit R&D Cat# DY1865-05 Mouse IFN-g DuoSet ELISA kit R&D Cat# DY485-05 Mouse IL-18 ELISA kit MBL Cat# 7625 Avidin/ Biotin Blocking Kit Vector Laboratories Cat# SP-2001 RRID:AB_2336231 Human Granzyme B DuoSet ELISA kit R&D Cat# DY2906-05 RRID:AB_2893368 Human Total IL-18 DuoSet ELISA kit R&D Cat# DY318-05 Experimental models: Cell lines Vero Cells (CCL81) ATCC CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Mouse: B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson Laboratory Cat# 022071; RRID:IMSR_JAX:022071 Mouse: Ncr1-Cre Provided by W. Yokoyama; Narni-Mancinelli et al.91 N/A Mouse: Il-18r1flox/flox Provided by R. A. Flavell; Nowarski et al.92 N/A Mouse: B6.129S2-Gzmbtm1Ley/J Provided by T. J. Ley; Pham et al.55 RRID:IMSR_JAX:002248 Mouse: C57BL/6-Prf1tm1Sdz/J Jackson Laboratory Cat# 002407; RRID:IMSR_JAX:002407 Software and algorithms Image J Fiji NIH https://imagej.net/software/fiji/ NDP 2.0 Hamamatsu https://www.hamamatsu.com/jp/en/ product/life-science-and-medical-systems/ digital-slide-scanner/U12388-01.html GraphPad Prism GraphPad Software http://www.graphpad.com/ scientific-software/prism/ FlowJo BD https://www.flowjo.com/solutions/flowjo Cell Reports 42, 112410, April 25, 2023 15 ..

    Incubation:

    Article Title: 40 Hz light stimulation restores early brain dynamics alterations and associative memory in Alzheimer’s disease model mice
    Article Snippet: Sections were blocked for one hour in 5% normal horse serum (NHS) diluted in PBS containing 0.5% Triton X- 100, followed by an 18-hour incubation at 4°C with mouse anti-6E10 antibody (1:1000 in 2% NHS, BioLegend #803001). .. After three PBS washes, sections were incubated for two hours with a biotinylated horse anti-mouse antibody (1:500 in PBS containing 0.5% Triton X-100, Vector Laboratories, #BA-2001-.5), followed by three PBS washes. .. Sections were then incubated for 30 minutes in an avidin-biotin solution (Vector Laboratories), washed twice in PBS, and finally in PB- Tris.

    Article Title: Neoantigens and uses thereof for treating cancer
    Article Snippet: The detection was performed with Streptavidin-HRP D (part of DABMap kit, Ventana Medical Systems), followed by incubation with Tyramide Alexa 488 (Invitrogen, cat #T20922) prepared according to manufacturer instruction with predetermined dilutions. .. Next, slides were incubated with anti-FoxP3 (Abcam, cat #ab20034, 5 ug/ml) for 4 hours, followed by 60 minutes incubation with biotinylated horse anti-mouse IgG (Vector Labs, cat #MKB-22258) at 1:200 dilution. .. The detection was performed with Streptavidin-HRP D (part of DABMap kit, Ventana Medical Systems), followed by incubation with Tyramide Alexa Fluor 568 (Invitrogen, cat #T20914) prepared according to manufacturer instruction with predetermined dilutions.

    Article Title: 40 Hz light stimulation restores early brain dynamics alterations and associative memory in Alzheimer’s disease model mice
    Article Snippet: Sections were blocked for 1 hour in 5% normal horse serum (NHS) diluted in PBS containing 0.5% Triton X-100, followed by an 18-hour incubation at 4°C with mouse anti-6E10 antibody (1:1000 in 2% NHS, BioLegend #803001). .. After three PBS washes, sections were incubated for 2 hours with a biotinylated horse anti-mouse antibody (1:500 in PBS containing 0.5% Triton X-100, Vector Laboratories, #BA-2001-.5), followed by three PBS washes. .. Sections were then incubated for 30 minutes in an avidin–biotin solution (Vector Laboratories), washed twice in PBS, and finally in PB-Tris.



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    Image Search Results


    Genetic knockout (KO) of inducible nitric oxide synthase (iNOS) results in a substantial reduction in the S-nitrosylation (SNO) of mitochondrial proteins . A-B. The images and the quantitated values of Western blots showing the total abundance of the representative mitochondrial proteins of each electron transport chain complex, in the heart tissues of wild-type (WT), VCP transgenic (TG), and VCP TG/iNOS KO mice. GAPDH used as a loading control in total protein extracts. C-D . The representative images and quantitated values of Western blots showing the SNO level of these proteins by testing the purified biotinylated proteins from the same heart tissues. *,p < 0.05, **, p < 0.01 vs. WT, ##: p < 0.01 vs. VCP TG mice.

    Journal: Redox Biology

    Article Title: Valosin-containing protein acts as a target and mediator of S-nitrosylation in the heart through distinct mechanisms

    doi: 10.1016/j.redox.2024.103166

    Figure Lengend Snippet: Genetic knockout (KO) of inducible nitric oxide synthase (iNOS) results in a substantial reduction in the S-nitrosylation (SNO) of mitochondrial proteins . A-B. The images and the quantitated values of Western blots showing the total abundance of the representative mitochondrial proteins of each electron transport chain complex, in the heart tissues of wild-type (WT), VCP transgenic (TG), and VCP TG/iNOS KO mice. GAPDH used as a loading control in total protein extracts. C-D . The representative images and quantitated values of Western blots showing the SNO level of these proteins by testing the purified biotinylated proteins from the same heart tissues. *,p < 0.05, **, p < 0.01 vs. WT, ##: p < 0.01 vs. VCP TG mice.

    Article Snippet: The biotinylated protein was probed with an anti-biotin antibody (1:3000) (Vector Laboratories, Burlingame, CA) and visualized with an enhanced chemiluminescent substrate (PerkinElmerLife Science).

    Techniques: Knock-Out, Western Blot, Transgenic Assay, Control, Purification