biotinylated horse anti mouse igg  (Vector Laboratories)

Journal: Redox Biology

Article Title: Valosin-containing protein acts as a target and mediator of S-nitrosylation in the heart through distinct mechanisms

doi: 10.1016/j.redox.2024.103166

Figure Lengend Snippet: Genetic knockout (KO) of inducible nitric oxide synthase (iNOS) results in a substantial reduction in the S-nitrosylation (SNO) of mitochondrial proteins . A-B. The images and the quantitated values of Western blots showing the total abundance of the representative mitochondrial proteins of each electron transport chain complex, in the heart tissues of wild-type (WT), VCP transgenic (TG), and VCP TG/iNOS KO mice. GAPDH used as a loading control in total protein extracts. C-D . The representative images and quantitated values of Western blots showing the SNO level of these proteins by testing the purified biotinylated proteins from the same heart tissues. *,p < 0.05, **, p < 0.01 vs. WT, ##: p < 0.01 vs. VCP TG mice.

Article Snippet: The biotinylated protein was probed with an anti-biotin antibody (1:3000) (Vector Laboratories, Burlingame, CA) and visualized with an enhanced chemiluminescent substrate (PerkinElmerLife Science).

Techniques: Knock-Out, Western Blot, Transgenic Assay, Control, Purification